The Last Supper is the final meal that, in the Gospel accounts, Jesus shared with his apostles in Jerusalem before his crucifixion. The Last Supper is commemorated by Christians especially on Holy Thursday. The Last Supper provides the scriptural basis for the Eucharist, also known as "Holy Communion" or "The Lord's Supper".

The First Epistle to the Corinthians contains the earliest known mention of the Last Supper. The four canonical gospels state that the Last Supper took place in the week of Passover, days after Jesus's triumphal entry into Jerusalem, and before Jesus was crucified on Good Friday. During the meal, Jesus predicts his betrayal by one of the apostles present, and foretells that before the next morning, Peter will thrice deny knowing him.

The three Synoptic Gospels and the First Epistle to the Corinthians include the account of the institution of the Eucharist in which Jesus takes bread, breaks it and gives it to those present, saying "This is my body given to you". The Gospel of John tells of Jesus washing the feet of the apostles,^{[John 13:1–15]} giving the new commandment "to love one another as I have loved you",^{[John 13:33–35]} and has a detailed farewell discourse by Jesus, calling the apostles who follow his teachings "friends and not servants", as he prepares them for his departure.^{[John 14–17]}

Some scholars have looked to the Last Supper as the source of early Christian Eucharistic traditions. Others see the account of the Last Supper as derived from 1st-century eucharistic practice as described by Paul in the mid-50s.